Friday, June 2, 2017

NEAUVIA STIMULATE SAFETY: IN VITRO STRESS TEST


Injectable medical devices are products that are in direct contact with
 the patient's skin and mucosa for extended periods of time.
The complete absence of systemic and, first and foremost, direct cellular
toxicity is the essential requirement for a filler that is implanted in the skin
 and remains there for 8 months (on average).
For this reason in particular, in order to exclude even the lowest of cytotoxic
effects, increasingly sensitive cytotoxicity assays are needed - which
 means performing in vitro tests using immortalised human keratinocytes as cell line.
Cytotoxicity can be assessed with the MTT test, which relies on mitochondrial
 activity. The test, originally developed by Mosman in 1983, is simple,
ccurate and yields reproducible results (Mosman, 1983). The MTT test is
 based on the principle that, in most viable cells, mitochondrial activity is
 constant, so an increase or decrease in the number of cells is directly
 proportional to mitochondrial activity. Viable cells convert MTT tetrazolium
 salts into formazan crystals, which can be solubilised in dimethyl sulphoxide
 (DMSO) for homogeneous spectrophotometric measurement.
Therefore any change in the number of viable cells can be detected by
 measuring the optical density of formazan at 570 nm.
NEAUVIA STIMULATE Hydrogel 26 mg of HA with 1 % hydroxyapatite
 has shown complete absence of cytotoxicity at various concentrations,
 even ones higher than the normal concentration used for clinical purposes.
NEAUVIA STIMULATE is a "hybrid" filler, new, safe and reversible,
which concentrates in a single product both the tissue filling functions
of a filler and the collagen production of particle fillers, which already
 have successfully been on the market for years. The amount of collagen
produced by NEAUVIA STIMULATE is comparable to particle fillers,
 without having the problem of being irreversibile. NEAUVIA STIMULATE
 is recommended for interventions on hands, cheeks, nasoslabial
and jaw area, and the aesthetic corrections last approximately 12 months.
Prime Journal

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